TCO (tetrazine - trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefin. Sulfo-Cyanine 5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation based labeling. This reagent possesses good aqueous solubility and stability in biological environments.
Absorption Maxima
646 nm
Extinction Coefficient
271000 M-1cm-1
Emission Maxima
662 nm
Fluorescence Quantum Yield
0.28
CF260
0.04
CF280
0.04
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C42H46KN7O7S2
Molecular Weight
864.09 kDa
Product Form
Dark blue solid.
Solubility
Good in water, DMF, and DMSO.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
