TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore has been used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM). Just like many other xanthene fluorophores, TAMRA is a available as two isomers (5- and 6-isomer) which have nearly identical optical properties. This product is an isomerically pure 5-TAMRA. TAMRA NHS is an amine reactive reagent. It can be used for the labeling of proteins, peptides, and modified oligonucleotides containing amine groups.
Absorption Maxima
541 nm
Extinction Coefficient
84000 M-1cm-1
Emission Maxima
567 nm
Fluorescence Quantum Yield
0.1
CAS Number
321862-17-3
CF260
0.32
CF280
0.19
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C29H25N3O7
Molecular Weight
527.53 kDa
Product Form
Dark colored solid.
Solubility
Good in DMF and DMSO. Low in water.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.