BLNK (phospho Tyr96) Cell Based ELISA Kit (A102662)

BLNK (phospho Tyr96) Cell Based ELISA Kit

Cell-based (qualitative)

This BLNK (phospho Tyr96) Cell Based ELISA Kit allows for the detection of BLNK (phospho Tyr96) and the effects that certain stimulation conditions have on BLNK (phospho Tyr96) expression in different cell lines. Qualitative determination of BLNK (phospho Tyr96) concentration is achieved by an indirect ELISA format. In essence, the BLNK (phospho Tyr96) is captured by Anti-BLNK (phospho Tyr96) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BLNK (phospho Tyr96) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-BLNK Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, B-cell linker protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa

Information online is representative. Always refer to the Product Manual inside the kit.