Bovine Antidiuretic Hormone ELISA Kit is a 60 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine Antidiuretic Hormone in serum, plasma, tissue homogenates, and other biological fluids.
Assay Type
Competitive (quantitative)
Principle of Assay
Bovine Antidiuretic Hormone ELISA Kit (A87434) is a 1 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine Antidiuretic Hormone in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Antidiuretic Hormone. During the assay, Antidiuretic Hormone present in the standards or samples competes with a fixed amount of assay-specific detection reagent for binding to the immobilized antibody. Following incubation, unbound components are removed by washing and HRP-conjugated detection reagent is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of Antidiuretic Hormone in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.469 pg/mL
Product Range
0.781-50 pg/mL
Assay Time
1 hour
Reactivity
Bovine
Cross Reactivity
Specific for Antidiuretic Hormone with no reported cross-reactivity with other analogs or variants.
Recovery
Sample Type
n
Range
Average
Serum
10
81% - 96%
86%
EDTA Plasma
10
81% - 95%
87%
Heparin Plasma
10
84% - 100%
93%
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
81-103%
84-101%
85-95%
EDTA Plasma
10
81-102%
81-99%
83-97%
Heparin Plasma
10
87-105%
81-99%
88-100%
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Synonyms
ADH, Arginine vasopressin neurophysin II, ARVP, AVP, AVP NPII, copeptin, Vasopressin, Vasopressin neurophysin II copeptin, VP
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Bovine Antidiuretic Hormone ELISA Kit (A87434) across serial sample dilutions. Linearity was evaluated using Serum, EDTA Plasma, and Heparin Plasma samples serially diluted from 1:2 to 1:8. Recovery (%) was calculated relative to the expected Bovine Antidiuretic Hormone concentration at each dilution. Data are presented as mean recovery values from 10 replicates (n = 10).
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