BRSK1 Cell Based ELISA Kit (A103487)

BRSK1 Cell Based ELISA Kit

Cell-based (qualitative)

This BRSK1 Cell Based ELISA Kit allows for the detection of BRSK1 and the effects that certain stimulation conditions have on BRSK1 expression in different cell lines. Qualitative determination of BRSK1 concentration is achieved by an indirect ELISA format. In essence, the BRSK1 is captured by Anti-BRSK1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BRSK1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

BR serine/threonine-protein kinase 1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, EC:2.7.11.26, hSAD1, KIAA1811, SAD1, SAD1 homolog, SADB, Serine/threonine-protein kinase BRSK1, Serine/threonine-protein kinase SAD-B, Synapses of Amphids Defective homolog 1

Information online is representative. Always refer to the Product Manual inside the kit.