BTK (phospho Tyr223) Cell Based ELISA Kit (A103824)

BTK (phospho Tyr223) Cell Based ELISA Kit

Cell-based (qualitative)

This BTK (phospho Tyr223) Cell Based ELISA Kit allows for the detection of BTK (phospho Tyr223) and the effects that certain stimulation conditions have on BTK (phospho Tyr223) expression in different cell lines. Qualitative determination of BTK (phospho Tyr223) concentration is achieved by an indirect ELISA format. In essence, BTK (phospho Tyr223) is captured by Anti-BTK (phospho Tyr223) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-BTK Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Fluorometric

> 5000 Cells

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Agammaglobulinemia tyrosine kinase, AGMX1, ATK, B-cell progenitor kinase, BPK, Bruton tyrosine kinase, Tyrosine-protein kinase BTK

Information online is representative. Always refer to the Product Manual inside the kit.