Principle of Assay
Canine Anti-KLH IgG ELISA Kit (A319521) employs the indirect sandwich enzyme immunoassay technique for the quantitative measurement of canine Anti-KLH IgG in serum, plasma, tissue homogenates, and other biological fluids. A capture antibody specific for Anti-KLH IgG is pre-coated onto the 96-well microtiter plate. Samples and standards are added, and Anti-KLH IgG present in each sample binds to the immobilized antibody. After washing, a Biotinylated detection antibody is added and incubated. Following washing, HRP-Streptavidin is added and incubated. TMB substrate is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Anti-KLH IgG captured, and the concentration is calculated by reading O.D. at 450nm and referring to the standard curve.
