Principle of Assay
Carnosine ELISA Kit (A2497) employs the competitive enzyme immunoassay technique for the quantitative measurement of Carnosine in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The 96-well microtiter plate has been pre-coated with Carnosine antigen. During the incubation, Carnosine present in the samples or standards competes with the fixed amount of immobilized Carnosine for binding sites on the Biotinylated Anti-Carnosine Antibody. The more Carnosine present in a sample or standard, the less Biotinylated Anti-Carnosine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Carnosine Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Carnosine present in each sample or standard. The concentration of Carnosine can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
