Caspase 8 (phospho Tyr380) Cell Based ELISA Kit (A102780)

Caspase 8 (phospho Tyr380) Cell Based ELISA Kit

Caspase 8 (phospho Tyr380) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of Caspase-8 levels in adherent and suspension cells.

Cell-based (qualitative)

This Caspase 8 (phospho Tyr380) Cell Based ELISA Kit allows for the detection of Caspase 8 (phospho Tyr380) and the effects that certain stimulation conditions have on Caspase 8 (phospho Tyr380) expression in different cell lines. Qualitative determination of Caspase 8 (phospho Tyr380) concentration is achieved by an indirect ELISA format. In essence, the Caspase 8 (phospho Tyr380) is captured by Anti-Caspase 8 (phospho Tyr380) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Caspase 8 (phospho Tyr380) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Caspase 8 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 3-6 months.

Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, MCH5

Information online is representative. Always refer to the Product Manual inside the kit.