CCRK Cell Based ELISA Kit (A103301)

CCRK Cell Based ELISA Kit

Cell-based (qualitative)

This CCRK Cell Based ELISA Kit allows for the detection of CCRK and the effects that certain stimulation conditions have on CCRK expression in different cell lines. Qualitative determination of CCRK concentration is achieved by an indirect ELISA format. In essence, the CCRK is captured by Anti-CCRK Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CCRK Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

CAK-kinase p42, CDCH, CDK-activating kinase p42, CDK20, Cell cycle-related kinase, Cell division protein kinase 20, Cyclin-dependent kinase 20, Cyclin-dependent protein kinase H, Cyclin-kinase-activating kinase p42

Information online is representative. Always refer to the Product Manual inside the kit.