CDC2L1 Cell Based ELISA Kit (A103417)

CDC2L1 Cell Based ELISA Kit

Cell-based (qualitative)

This CDC2L1 Cell Based ELISA Kit allows for the detection of CDC2L1 and the effects that certain stimulation conditions have on CDC2L1 expression in different cell lines. Qualitative determination of CDC2L1 concentration is achieved by an indirect ELISA format. In essence, the CDC2L1 is captured by Anti-CDC2L1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CDC2L1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

CDK11, Cell division cycle 2-like protein kinase 1, Cell division protein kinase 11B, CLK-1, Cyclin-dependent kinase 11B, Galactosyltransferase-associated protein kinase p58/GTA, p58 CLK-1, PITSLRE serine/threonine-protein kinase CDC2L1, PITSLREA, PK58

Information online is representative. Always refer to the Product Manual inside the kit.