DGKE Cell Based ELISA Kit (A103286)

DGKE Cell Based ELISA Kit

DGKE Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of DGKE levels in adherent and suspension cells.

Cell-based (qualitative)

This DGKE Cell Based ELISA Kit allows for the detection of DGKE and the effects that certain stimulation conditions have on DGKE expression in different cell lines. Qualitative determination of DGKE concentration is achieved by an indirect ELISA format. In essence, the DGKE is captured by Anti-DGKE Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this DGKE Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

DAG kinase epsilon, DAGK5, DGK-epsilon, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

Information online is representative. Always refer to the Product Manual inside the kit.