Fos Cell Based ELISA Kit (A103314)

Fos Cell Based ELISA Kit

Fos Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of c-Fos levels in adherent and suspension cells.

Cell-based (qualitative)

This Fos Cell Based ELISA Kit allows for the detection of Fos and the effects that certain stimulation conditions have on Fos expression in different cell lines. Qualitative determination of Fos concentration is achieved by an indirect ELISA format. In essence, the Fos is captured by Anti-Fos Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Fos Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

Cellular oncogene fos, FOS, Fos proto-oncogene, AP-1 transcription factor subunit, G0/G1 switch regulatory protein 7, G0S7, Protein c-Fos, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

Information online is representative. Always refer to the Product Manual inside the kit.