Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

Histone H1 (phospho Thr17) Cell Based ELISA Kit

Cell-based (qualitative)

This Histone H1 (phospho Thr17) Cell Based ELISA Kit allows for the detection of Histone H1 (phospho Thr17) and the effects that certain stimulation conditions have on Histone H1 (phospho Thr17) expression in different cell lines. Qualitative determination of Histone H1 (phospho Thr17) concentration is achieved by an indirect ELISA format. In essence, Histone H1 (phospho Thr17) is captured by Anti-Histone H1 (phospho Thr17) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-Histone H1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Microplate

> 5000 Cells

Human, Mouse

Store at + 4°C. Stable for 6 months.

H1-0, H1-1, H1-10, H1-3, H1-5, H1-6, H1-8, H1F0, H1F1, H1F3, H1F5, H1FOO, H1FT, H1FV, H1FX, H1OO, H1T, HIST1H1A, HIST1H1B, HIST1H1D, HIST1H1T, Histone H1', Histone H1(0), Histone H1.0, Histone H1.1, Histone H1.10, Histone H1.3, Histone H1.5, Histone H1.8, Histone H1a, Histone H1b, Histone H1c, Histone H1oo, Histone H1s-2, Histone H1s-3, Histone H1t, Histone H1x, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone

Information online is representative. Always refer to the Product Manual inside the kit.