HNF4 alpha Cell Based ELISA Kit (A102890)

HNF4 alpha Cell Based ELISA Kit

HNF4 alpha Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of HNF 4 alpha levels in adherent and suspension cells.

Cell-based (qualitative)

This HNF4 alpha Cell Based ELISA Kit allows for the detection of HNF4 alpha and the effects that certain stimulation conditions have on HNF4 alpha expression in different cell lines. Qualitative determination of HNF4 alpha concentration is achieved by an indirect ELISA format. In essence, the HNF4 alpha is captured by Anti-HNF4 alpha Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this HNF4 alpha Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, HNF4, HNF4A, NR2A1, Nuclear receptor subfamily 2 group A member 1, TCF-14, TCF14, Transcription factor 14, Transcription factor HNF-4

Information online is representative. Always refer to the Product Manual inside the kit.