Human AFP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human AFP in the samples. A monoclonal antibody specific for human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for AFP is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.45 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both natural and recombinant human AFP.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
98% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
