Human Alpha-fetoprotein ELISA Kit (A1543)

Human Alpha-fetoprotein ELISA Kit

Human Alpha-fetoprotein ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Alpha-fetoprotein in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human AFP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human AFP in the samples. A monoclonal antibody specific for human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for AFP is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

Colorimetric

0.45 pg/ml

15.63 - 1000 pg/ml

This kit can assay both native and recombinant human AFP.

Human

This ELISA kit has shown no significant cross-reactivity.

98% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Information online is representative. Always refer to the Product Manual inside the kit.