Human Angiopoietin 1 ELISA Kit (A1383)

Human Angiopoietin 1 ELISA Kit

Human Angiopoietin 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Angiopoietin-1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Angiopoietin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-1 in the samples. A monoclonal antibody specific for human ANG-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and ANG-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ANG-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.60 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human ANG-1.

Human

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

ANG-1, ANGPT1, KIAA0003

Information online is representative. Always refer to the Product Manual inside the kit.