Human Angiopoietin 2 ELISA Kit (A1384)

Human Angiopoietin 2 ELISA Kit

Human Angiopoietin 2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Angiopoietin-2 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Angiopoietin-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-2 in the samples. A monoclonal antibody specific for human ANG-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and ANG-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-2 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of ANG-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

Colorimetric

2.61 pg/ml

15.63 - 1000 pg/ml

This kit can assay both native and recombinant human ANG-2.

Human

This ELISA kit has shown no significant cross-reactivity.

92% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

ANG-2, ANGPT2

Information online is representative. Always refer to the Product Manual inside the kit.