Principle of Assay
Human Angiotensinogen ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human AGT in the samples. A monoclonal antibody specific for human AGT has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for AGT are pipetted into the wells and AGT present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of AGT bound in the initial step. The color development is stopped and the intensity of the color is measured.
