Human Apolipoprotein B48 ELISA Kit (A327026)

Human Apolipoprotein B48 ELISA Kit

Human Apolipoprotein B48 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of human Apolipoprotein B48 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Human Apolipoprotein B48 ELISA Kit (A327026) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Apolipoprotein B48 in serum, plasma, and other biological fluids. An antibody specific for Apolipoprotein B48 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Apolipoprotein B48 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Apolipoprotein B48 Antibody, which binds the captured Apolipoprotein B48 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Apolipoprotein B48 captured in each well. The concentration of Apolipoprotein B48 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.94 ng/ml

1.56-100 ng/ml

100µl

Human

No significant cross-reactivity or interference between human Apolipoprotein B48 and analogues was observed.

Intra-assay CV: 5.15%, Inter-assay CV: 4.7%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Apo B-100, Apo B-48, APOB, ApoB 100 , ApoB 48, APOB_HUMAN, Apolipoprotein B, Apolipoprotein B (including Ag(x) antigen), Apolipoprotein B 100, Apolipoprotein B-48, FLDB, LDLCQ4

Information online is representative. Always refer to the Product Manual inside the kit.