Human Apolipoprotein J ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Apolipoprotein J in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Apolipoprotein J ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo J in the samples. A monoclonal antibody specific for human Apo J has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo J, and Apo J present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo J bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
2.08 ng/ml
Product Range
31.25 - 2000 ng/ml
Specificity
This kit can assay both native and recombinant human Apo J.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
Aging-associated gene 4 protein, Apo-J, APOJ, CLI, CLU, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, KUB1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
