Principle of Assay
Human ATP-binding Cassette Sub-family A Member 3 ELISA Kit (A310735) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human ATP-binding Cassette Sub-family A Member 3 in serum, plasma or other biological fluids. An antibody specific for ATP-binding Cassette Sub-family A Member 3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ATP-binding Cassette Sub-family A Member 3 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-ATP-binding Cassette Sub-family A Member 3 Antibody, which also binds the ATP-binding Cassette Sub-family A Member 3 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-ATP-binding Cassette Sub-family A Member 3 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-ATP-binding Cassette Sub-family A Member 3 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of ATP-binding Cassette Sub-family A Member 3 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of ATP-binding Cassette Sub-family A Member 3 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
