Human BAFF ELISA Kit (A1392)

Human BAFF ELISA Kit

Human BAFF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BAFF in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human BAFF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BAFF in the samples. A monoclonal antibody specific for human BAFF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BAFF, and BAFF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BAFF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human BAFF.

Human

This ELISA kit has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

B lymphocyte stimulator, B-cell-activating factor, BLYS, CD257, Dendritic cell-derived TNF-like molecule, TALL-1, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4

Information online is representative. Always refer to the Product Manual inside the kit.