Human beta Nerve Growth Factor ELISA Kit (A1544)

Human beta Nerve Growth Factor ELISA Kit

Human beta Nerve Growth Factor ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human beta Nerve Growth Factor in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human ß-NGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ß-NGF in the samples. A monoclonal antibody specific for human ß-NGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for ß-NGF, and ß-NGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ß-NGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.78 pg/ml

15.63 - 1000 pg/ml

This kit can assay both native and recombinant human ß-NGF.

Human

This ELISA kit has shown no significant cross-reactivity.

98% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID67

Information online is representative. Always refer to the Product Manual inside the kit.