Human BMP-2 ELISA Kit (A1395)

Human BMP-2 ELISA Kit

Human BMP-2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BMP-2 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human BMP-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-2 in the samples. A monoclonal antibody specific for human BMP-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BMP-2, and BMP-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BMP-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

1.73 pg/ml

15.63 - 1000 pg/ml

This kit can assay both native and recombinant human BMP-2.

Human

This ELISA kit has shown no significant cross-reactivity.

96% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

BMP-2A, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

Information online is representative. Always refer to the Product Manual inside the kit.