Human CD117 ELISA Kit (A1407)

Human CD117 ELISA Kit

Human CD117 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CD117 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human CD117 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CD117 in the samples. A monoclonal antibody specific for human CD117 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CD117, and CD117 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CD117 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.16 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human CD117.

Human

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

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Information online is representative. Always refer to the Product Manual inside the kit.