Human CD126 ELISA Kit (A341724)

Human CD126 ELISA Kit

Human CD126 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human CD126 in biological samples.

Sandwich (quantitative)

Human CD126 ELISA Kit (A341724) is a sandwich ELISA designed for the sensitive and quantitative measurement of human CD126 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for CD126. Standards and samples are added to the wells, allowing CD126 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for CD126 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of CD126 in the sample. The concentration of CD126 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

5 pg/ml

31.25-1000 pg/ml

1h 45m

This product recognizesboth natural and recombinant human soluble IL-6R.

Human

This product shows no cross reactivity with other human soluble receptors. No interference with neither soluble gp130 nor IL-6.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C

gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL6R, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80

Information online is representative. Always refer to the Product Manual inside the kit.