Human CD177 ELISA Kit (A329999)

Human CD177 ELISA Kit

Human CD177 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CD177 in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Human CD177 ELISA Kit (A329999) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CD177 in serum, plasma and cell culture supernates. An antibody specific for CD177 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD177 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CD177 Antibody, which binds the captured CD177 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CD177 captured in each well. The concentration of CD177 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

4.14 pg/ml

39.06-2500 pg/ml

4 hours

100µl

Human

Intra-assay CV: <4.1%, Inter-assay CV: <8.5%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-CD177 Antibody (A329202) as the capture antibody, Anti-CD177 Antibody (A329203) as the detection antibody, and Recombinant Human CD177 Protein (C-terminal Human Fc and His Tag) (A330257) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

CD177 antigen, HNA-2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, PRV1

Information online is representative. Always refer to the Product Manual inside the kit.