Principle of Assay
Human Anti-CD3 Antibody ELISA Kit (A326361) employs the Indirect ELISA technique for the quantitative measurement of human Anti-CD3 Antibody in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The 96-well microtiter plate has been pre-coated with Anti-CD3 Antibody antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of Anti-CD3 Antibody sample captured on the plate. The concentration of Anti-CD3 Antibody can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
