Human CD44 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human CD44 in biological samples.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human CD44 ELISA Kit (A341730) is a sandwich ELISA designed for the sensitive and quantitative measurement of human CD44 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for CD44. Standards and samples are added to the wells, allowing CD44 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for CD44 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of CD44 in the sample. The concentration of CD44 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
Platform
Pre-coated Microplate (1 x 96)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma and cell culture supernatants.
Sensitivity
0.113 ng/ml
Product Range
0.31-10 ng/ml
Assay Time
2h 15m
Specificity
This product recognizesboth natural and recombinant human soluble Pgp-1.
Reactivity
Human
Cross Reactivity
This product shows no cross reactivity with other human adhesion molecules.
General Notes
For bulk or custom IVD raw material requests, contact us directly. IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.
