Human CD54 ELISA Kit (A341727)

Human CD54 ELISA Kit

Human CD54 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human CD54 in biological samples.

Sandwich (quantitative)

Human CD54 ELISA Kit (A341727) is a sandwich ELISA designed for the sensitive and quantitative measurement of human CD54 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for CD54. Standards and samples are added to the wells, allowing CD54 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for CD54 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of CD54 in the sample. The concentration of CD54 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

0.1 ng/ml

0.25-8 ng/ml

1h 45m

This product recognizesboth natural and recombinant human soluble ICAM-1.

Human

This product shows no cross reactivity with other human adhesion molecules.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C

ICAM-1, ICAM1, Intercellular adhesion molecule 1, Major group rhinovirus receptor

Information online is representative. Always refer to the Product Manual inside the kit.