Human CD62P ELISA Kit (A341757)

Human CD62P ELISA Kit

Human CD62P ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human CD62P in biological samples.

Sandwich (quantitative)

Human CD62P ELISA Kit (A341757) is a sandwich ELISA designed for the sensitive and quantitative measurement of human CD62P in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for CD62P. Standards and samples are added to the wells, allowing CD62P present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for CD62P is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of CD62P in the sample. The concentration of CD62P is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

3.6 ng/ml

4.4-140 ng/ml

2h 45m

This product recognizesboth natural and recombinant human P-Selectin.

Human

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

CD62 antigen-like family member P, GMP-140, GMRP, Granule membrane protein 140, GRMP, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein, SELP

Information online is representative. Always refer to the Product Manual inside the kit.