Principle of Assay
Human Creatine Kinase MT ELISA Kit (A2368) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Creatine Kinase MT in serum, plasma, tissue homogenates or other biological fluids. An antibody specific for Creatine Kinase MT has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Creatine Kinase MT present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Creatine Kinase MT Antibody, which binds the captured Creatine Kinase MT present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Creatine Kinase MT captured in each well. The concentration of Creatine Kinase MT can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
