Human CXCL13 ELISA Kit (A1416)

Human CXCL13 ELISA Kit

Sandwich (quantitative)

Human CXCL13 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CXCL13 in the samples. A monoclonal antibody specific for human CXCL13 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CXCL13, and CXCL13 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL13 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human CXCL13.

Human

This assay has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, BCA-1, BCA1, BLC, C-X-C motif chemokine 13, CXC chemokine BLC, SCYB13, Small-inducible cytokine B13

Information online is representative. Always refer to the Product Manual inside the kit.