Human Cytomegalovirus IgG ELISA Kit is a 90 minute indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Cytomegalovirus IgG in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
Assay Type
Indirect (qualitative)
Principle of Assay
Human Cytomegalovirus IgG ELISA Kit (A351756) is a 1h 30m indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Cytomegalovirus IgG in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
Sensitivity
0.938 ng/mL
Product Range
1.563-100 ng/mL
Assay Time
1h 30m
Reactivity
Human
Cross Reactivity
Specific for Cytomegalovirus IgG with no reported cross-reactivity with other analogs or variants.
Recovery
Sample Type
n
Range
Average
Serum
10
86% - 103%
94%
EDTA Plasma
10
88% - 104%
96%
Heparin Plasma
10
93% - 102%
98%
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
90-105%
86-104%
87-105%
EDTA Plasma
10
86-97%
87-97%
84-95%
Heparin Plasma
10
82-100%
85-97%
81-95%
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Human Cytomegalovirus IgG ELISA Kit (A351756) across serial sample dilutions. Linearity was evaluated using Serum, EDTA Plasma, and Heparin Plasma samples serially diluted from 1:2 to 1:8. Recovery (%) was calculated relative to the expected Human Cytomegalovirus IgG concentration at each dilution. Data are presented as mean recovery values from 10 replicates (n = 10).
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