Principle of Assay
Human Enterovirus 71 IgM ELISA Kit (A247206) is a 3 hour trapping Enzyme-Linked Immunosorbent Assay (tELISA) designed for the in vitro qualitative detection of human Enterovirus 71 IgM in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an assay-specific trapping reagent. Samples are added to the wells, where Enterovirus 71 IgM is captured by the immobilized trapping reagent. Following incubation, unbound components are removed by washing and an assay-specific detection reagent is added. This detection reagent binds the captured Enterovirus 71 IgM to form a trapped immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The presence or absence of colour indicates the presence or absence of Enterovirus 71 IgM in the sample.
