Human ErbB2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ErbB2 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human ErbB2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ErbB2 in the samples. A monoclonal antibody specific for human ErbB2 has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for ErbB2 are pipetted into the wells and ErbB2 present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of ErbB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.51 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both native and recombinant human ErbB2.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.