Principle of Assay
This Human esRAGE ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human esRAGE in plasma, tissue homogenates and other biological fluids.
An antibody specific for esRAGE has been coated on a microplate. Standards and samples are pipetted into the wells and the esRAGE present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-esRAGE Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of esRAGE bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
