Principle of Assay
Human Factor H ELISA Kit (A335047) is an Indirect ELISA for the quantitative measurement of human Factor H in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The 96-well microtiter plate has been pre-coated with Factor H antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of Factor H sample captured on the plate. The concentration of Factor H can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
