Human FAK ELISA Kit (A8093)

Human FAK ELISA Kit

Human FAK ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human FAK in tissue homogenates or other biological fluids.

Sandwich (quantitative)

Human FAK ELISA Kit (A8093) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human FAK in tissue homogenates or other biological fluids. An antibody specific for FAK has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the FAK present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-FAK Antibody, which binds the captured FAK present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of FAK captured in each well. The concentration of FAK can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Tissue homogenates or other biological fluids.

0.53 ng/ml

1.56-100 ng/ml

4h 30m

Human

Intra-assay: CV < 10%, Inter-assay: CV < 12%

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

FADK 1, FAK1, Focal adhesion kinase 1, Focal adhesion kinase-related nonkinase, FRNK, p125FAK, pp125FAK, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, PTK2

Information online is representative. Always refer to the Product Manual inside the kit.