Human Fas ELISA Kit (A1428)

Human Fas ELISA Kit

Human Fas ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Fas in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Fas ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Fas in the samples. A monoclonal antibody specific for human Fas has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Fas, and Fas present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Fas bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

3.15 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human Fas.

Human

This ELISA kit has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

Apo-1 antigen, Apoptosis-mediating surface antigen FAS, APT1, CD95, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

Information online is representative. Always refer to the Product Manual inside the kit.