Human Galectin-3 ELISA Kit (A1435)

Human Galectin-3 ELISA Kit

Human Galectin-3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Galectin-3 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Galectin-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Galectin-3 in the samples. A monoclonal antibody specific for human Galectin-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Galectin-3, and Galectin-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Galectin-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.46 pg/ml

62.50 - 4000 pg/ml

This kit can assay both native and recombinant human Galectin-3.

Human

This ELISA kit has shown no significant cross-reactivity.

101% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, LGALS3, MAC2

Information online is representative. Always refer to the Product Manual inside the kit.