Human Galectin-3 ELISA Kit (A1435)

Human Galectin-3 ELISA Kit

Sandwich (quantitative)

Human Galectin-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Galectin-3 in the samples. A monoclonal antibody specific for human Galectin-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Galectin-3, and Galectin-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Galectin-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

This kit can assay both natural and recombinant human Galectin-3.

Human

This assay has shown no significant cross-reactivity.

101% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, LGALS3, MAC2

Information online is representative. Always refer to the Product Manual inside the kit.