Human GDF-15 ELISA Kit (A1436)

Human GDF-15 ELISA Kit

Sandwich (quantitative)

Human GDF-15 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GDF-15 in the samples. A monoclonal antibody specific for human GDF-15 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for GDF-15, and GDF-15 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of GDF-15 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

15.63 - 1000 pg/ml

This kit can assay both natural and recombinant human GDF-15.

Human

This assay has shown no significant cross-reactivity.

97% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC-1, MIC1, NAG-1, NRG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PTGFB

Information online is representative. Always refer to the Product Manual inside the kit.