Human GPx-7 ELISA Kit (A303296)

Human GPx-7 ELISA Kit

Human GPx-7 ELISA Kit is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GPx-7 in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Human GPx-7 ELISA Kit (A303296) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GPx-7 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for GPx-7. Standards and samples are added to the wells, where GPx-7 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for GPx-7 is added to the wells. The detection antibody binds the captured GPx-7 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of GPx-7 captured in each well. The concentration of GPx-7 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

0.094 ng/mL

4 hours

Human

Specific for GPx-7 with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

CL683, Glutathione peroxidase 7, GPX6, GSHPx-7

Information online is representative. Always refer to the Product Manual inside the kit.