Principle of Assay
Human Hepatitis B Virus Surface Antigen ELISA Kit (A76052) employs an enzyme immunoassay for the qualitative detection of human Hepatitis B Virus Surface Antigen in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate is coated with capture antibodies or antigens specific for Hepatitis B Virus Surface Antigen. Samples are added and incubated, followed by washing and addition of enzyme-conjugated detection molecules. TMB substrate is added to produce a blue-coloured product that turns yellow after acidic stop solution. The presence or absence of yellow color indicates the presence or absence of Hepatitis B Virus Surface Antigen in the sample.