Human Anti-Hepatitis C Virus Antibody ELISA Kit (A74301)

Human Anti-Hepatitis C Virus Antibody ELISA Kit

Human Anti-Hepatitis C Virus Antibody ELISA Kit is a 120 minute qualitative Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro qualitative determination of human Anti-Hepatitis C Virus Antibody in serum, plasma, tissue homogenates, and other biological fluids.

Indirect (qualitative)

Human Anti-Hepatitis C Virus Antibody ELISA Kit (A74301) is a 2 hour qualitative indirect Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro quantitative determination of human Anti-Hepatitis C Virus Antibody in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-Hepatitis C Virus Antibody present in the samples binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-Hepatitis C Virus Antibody present in the sample. The concentration of Anti-Hepatitis C Virus Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

2 hours

Human

Demonstrates specificity for anti-Hepatitis C Virus antibodies with no reported cross-reactivity to related analogs.

This qualitative ELISA provides only a positive or negative result and does not measure or quantify the concentration of the analyte in the sample.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.