Principle of Assay
Human Herpes Simplex Virus 2 IgG ELISA Kit (A303135) employs an enzyme immunoassay for the qualitative detection of human Herpes Simplex Virus 2 IgG in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate is coated with capture antibodies or antigens specific for Herpes Simplex Virus 2 IgG. Samples are added and incubated, followed by washing and addition of enzyme-conjugated detection molecules. TMB substrate is added to produce a blue-coloured product that turns yellow after acidic stop solution. The presence or absence of yellow color indicates the presence or absence of Herpes Simplex Virus 2 IgG in the sample.