Human HSD17B1 ELISA Kit (A302888)

Human HSD17B1 ELISA Kit

Human HSD17B1 ELISA Kit is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HSD17B1 in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Human HSD17B1 ELISA Kit (A302888) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HSD17B1 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for HSD17B1. Standards and samples are added to the wells, where HSD17B1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for HSD17B1 is added to the wells. The detection antibody binds the captured HSD17B1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of HSD17B1 captured in each well. The concentration of HSD17B1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

0.094 ng/mL

4 hours

Human

Specific for HSD17B1 with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

17-beta-HSD 1, 17-beta-hydroxysteroid dehydrogenase type 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, SDR28C1, Short chain dehydrogenase/reductase family 28C member 1

Information online is representative. Always refer to the Product Manual inside the kit.