Principle of Assay
Human IFN-a ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IFN-a in the samples. A monoclonal antibody specific for human IFN-a has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of HRP-linked detect antibody specific for IFN-a, and IFN-a present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, substrate solution reacts with HRP and color develops in proportion to the amount of IFN-a bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
