Human IFN gamma ELISA Kit (A341794)

Human IFN gamma ELISA Kit

Human IFN gamma ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human IFN gamma in biological samples.

Sandwich (quantitative)

Human IFN gamma ELISA Kit (A341794) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IFN gamma in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IFN gamma. Standards and samples are added to the wells, allowing IFN gamma present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IFN gamma is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IFN gamma in the sample. The concentration of IFN gamma is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

5 pg/ml

12.5-400 pg/ml

2h 45m

This product recognizesboth natural and recombinant human IFN-g.

Human

This product shows no cross reactivity with other human cytokines. Cross reactivity with simian IFN gamma.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

IFN-gamma, IFNG, Immune interferon, Interferon gamma

Information online is representative. Always refer to the Product Manual inside the kit.